3GYY
The ectoine binding protein of the TeaABC TRAP transporter TeaA in the Apo-State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Collection date | 2008-12-01 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.740, 64.980, 121.500 |
| Unit cell angles | 90.00, 91.06, 90.00 |
Refinement procedure
| Resolution | 47.566 - 2.200 |
| R-factor | 0.2201 |
| Rwork | 0.217 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vpn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.817 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.566 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.214 | 0.900 |
| Number of reflections | 57867 | 7165 |
| <I/σ(I)> | 8.03 | 2.22 |
| Completeness [%] | 97.3 | 97.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 100mM Tris, 50mM zinc acetate, 30% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
