3GYV
Crystal structure of nucleosome assembly protein from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.680, 37.830, 79.310 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.217 |
| R-free | 0.30700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.224 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.084 | 0.331 |
| Number of reflections | 4580 | |
| <I/σ(I)> | 45.6 | 11.4 |
| Completeness [%] | 99.0 | 97.6 |
| Redundancy | 14.5 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 20% PEG 3350, 0.2M magnesium chloride, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
