3GYR
Structure of Phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center.
Replaces: 2G23Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-22 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 109.494, 163.456, 164.352 |
| Unit cell angles | 117.04, 95.74, 107.23 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.16743 |
| Rwork | 0.165 |
| R-free | 0.22237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gsk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.585 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.400 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.400 |
| Number of reflections | 357511 | |
| <I/σ(I)> | 11.6 | 2.6 |
| Completeness [%] | 86.3 | 72.4 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 278 | PEG 4000, SODIUM CITRATE, 2- PROPANOL, GLYCEROL, PH 5.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 288K, PH 5.00, temperature 278K |
