3GYJ
Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.289, 73.396, 63.140 |
| Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
| Resolution | 36.700 - 0.920 |
| R-factor | 0.1248 |
| Rwork | 0.124 |
| R-free | 0.15140 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 0.940 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.048 | 0.370 |
| Number of reflections | 291838 | |
| <I/σ(I)> | 25.5 | 3.03 |
| Completeness [%] | 92.7 | 88 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 9-11% PEG 8000, 75 mM MnSO4, 100 mM cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
