3GYI
Cholesterol oxidase from Streptomyces sp. N485D mutant (1.0A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.276, 72.783, 62.970 |
| Unit cell angles | 90.00, 105.12, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.000 |
| R-factor | 0.126 |
| Rwork | 0.126 |
| R-free | 0.15760 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.069 | 0.543 |
| Number of reflections | 231276 | |
| <I/σ(I)> | 21.7 | 1.5 |
| Completeness [%] | 96.2 | 58.9 |
| Redundancy | 4.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 9-11% PEG 8000, 75 mM MnSO4, 100 mM cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
