3GXX
Structure of the SH2 domain of the Candida glabrata transcription elongation factor Spt6, crystal form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97971 |
| Spacegroup name | P 32 |
| Unit cell lengths | 71.600, 71.600, 87.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.700 - 2.400 |
| Rwork | 0.253 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gxw |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 18513 | |
| <I/σ(I)> | 45.1 | 7.7 |
| Completeness [%] | 99.5 | 95.4 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM bicine pH 8.0, 4,3 M NaCl and 5 mM Tris(2-Carboxyethyl) phosphine Hydro-chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
