3GXW

Structure of the SH2 domain of the Candida glabrata transcription elongation factor Spt6, crystal form A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97973, 0.97987, 0.90810
Spacegroup name	P 65
Unit cell lengths	54.499, 54.499, 253.434
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.196
R-free	0.24100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	33162
<I/σ(I)>	42.9	7.3
Completeness [%]	99.4	96.6
Redundancy	4.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM HEPES pH 7.0, 1 M succinic acid, 1% (v/v) PEG 2000 MME, 5 mM Tris(2-Carboxyethyl) phosphine hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K