3GX8
Structural and biochemical characterization of yeast monothiol glutaredoxin Grx5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.120, 85.120, 48.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.214 - 1.673 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yka |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.982 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.570 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.034 | 0.303 |
| Number of reflections | 15256 | |
| <I/σ(I)> | 21.7 | 5.4 |
| Completeness [%] | 98.5 | 89.6 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 1.6M NH4SO4, Tris-HCl, pH8.0, VAPOR DIFFUSION, temperature 289K |
