3GWJ

Crystal structure of Antheraea pernyi arylphorin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	20
Detector technology	CCD
Detector	ADSC QUANTUM 4r
Spacegroup name	P 21 21 21
Unit cell lengths	121.196, 119.470, 319.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.430
R-factor	0.182
Rwork	0.182
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcy
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.420
Number of reflections	168198

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M sodium acetate, 14.5% PEG 3350, 0.15M magnesium formate, 20mM benzamidine chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M sodium acetate, 14.5% PEG 3350, 0.15M magnesium formate, 20mM benzamidine chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M sodium acetate, 14.5% PEG 3350, 0.15M magnesium formate, 20mM benzamidine chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K