3GWG
Crystal structure of CheY of Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.320, 38.476, 38.856 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 19.370 - 1.800 |
| R-factor | 0.14861 |
| Rwork | 0.147 |
| R-free | 0.18609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3chy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.308 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.160 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.031 | 0.072 |
| Number of reflections | 9175 | |
| <I/σ(I)> | 30.3 | 15.4 |
| Completeness [%] | 98.1 | 96.1 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 1.6M ammonium sulfate, 0.1M sodium acetate, 35% MPEG2000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
