3GWF
Open crystal structure of cyclohexanone monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 93 |
| Collection date | 2004-09-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.279, 66.958, 135.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.18767 |
| Rwork | 0.182 |
| R-free | 0.23666 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w4x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.325 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.317 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 27243 | |
| Completeness [%] | 99.3 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 0.2M magnesium acetate tetrahydrate, 0.1M sodium cacodylate pH 6.5 and 20% PEG 8000, VAPOR DIFFUSION |
