3GWA
1.6 Angstrom crystal structure of 3-oxoacyl-(acyl-carrier-protein) synthase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.260, 80.260, 193.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 1.600 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zow |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 110601 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.0 | 96.6 |
| Redundancy | 12.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 100MM SUCCINIC ACID, 15% PEG 3350, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K 50 mg/ml protein |
