3GW4

Crystal structure of uncharacterized protein from Deinococcus radiodurans. Northeast Structural Genomics Consortium Target DrR162B.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97914, 0.97928, 0.96784
Spacegroup name	P 21 21 21
Unit cell lengths	57.850, 70.680, 102.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.430 - 2.490
R-factor	0.227
Rwork	0.225
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.199
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.490	2.490
Rmerge	0.100	0.434
Number of reflections	15135
<I/σ(I)>	25.4	6.7
Completeness [%]	98.4	98.5
Redundancy	4.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5. Reservoir solution: 1.9M Na-malonate, VAPOR DIFFUSION, HANGING DROP, temperature 294K