3GW1

The structure of the Caulobacter crescentus CLPs protease adaptor protein in complex with FGG tripeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	27.599, 38.320, 62.857
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	32.719 - 2.360
R-factor	0.2717
Rwork	0.270
R-free	0.29740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dnj
RMSD bond length	0.004
RMSD bond angle	0.670
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.440
High resolution limit [Å]	2.360	6.510	2.360
Rmerge	0.110	0.047	0.318
Number of reflections	4895
<I/σ(I)>	12.933
Completeness [%]	96.1	95.3	96.8
Redundancy	2.8	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	300	0.1 M bis-tris pH 5.5, 0.2 M MgCl2, 25% PEG 3350, vapor diffusion, temperature 300K