3GVZ
Crystal structure of the protein CV2077 from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.096, 87.053, 115.120 |
| Unit cell angles | 90.00, 100.82, 90.00 |
Refinement procedure
| Resolution | 19.620 - 2.800 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 | 0.110 |
| Number of reflections | 31015 | |
| <I/σ(I)> | 13.69 | 6.16 |
| Completeness [%] | 76.6 | 52.3 |
| Redundancy | 2.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6.5 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5. Reservoir solution: 0.1M MES pH 6.5, 20% PEG 10000, MICROBATCH UNDER OIL, temperature 291K |
