3GVM

Structure of the homodimeric WXG-100 family protein from Streptococcus agalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	133.100, 132.530, 43.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.957 - 2.150
R-factor	0.185
Rwork	0.183
R-free	0.21500
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Auto-Rickshaw
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.091	0.507
Number of reflections	43011	3128
<I/σ(I)>		3.8
Completeness [%]	99.9	99.9
Redundancy	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	0.1M Tris-HCl pH 8.0, 1.9M (NH4)SO4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K
1	VAPOR DIFFUSION, HANGING DROP	8	297	0.1M Tris-HCl pH 8.0, 1.9M (NH4)SO4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K