3GV8

Human DNA polymerase iota in complex with T template DNA and incoming dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2008-03-07
Spacegroup name	P 65 2 2
Unit cell lengths	98.070, 98.070, 203.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.840 - 2.000
R-factor	0.219
Rwork	0.218
R-free	0.24800
RMSD bond length	0.019
RMSD bond angle	1.821
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.121	0.083	0.577
Number of reflections	40059
<I/σ(I)>	57.94
Completeness [%]	99.7	97.1	100
Redundancy	13.3	12.7	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5		15% PEG 5000MME, 0.2M NH4SO4, 0.1M MES 6.5, 5% glycerol, hanging drop