3GV7

Human DNA polymerase iota in complex with T template DNA and incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2008-03-07
Spacegroup name	P 65 2 2
Unit cell lengths	97.190, 97.190, 201.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.040 - 2.200
R-factor	0.21
Rwork	0.209
R-free	0.25800
RMSD bond length	0.013
RMSD bond angle	1.557
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	27.040
High resolution limit [Å]	2.200
Number of reflections	29366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5		15% PEG 5000MME, 0.2M NH4SO4, 0.1M MES 6.5, 5% glycerol, hanging drop