3GV2
X-ray Structure of Hexameric HIV-1 CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.618, 156.437, 196.644 |
| Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
| Resolution | 49.754 - 7.000 |
| R-factor | 0.286 |
| Rwork | 0.282 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.483 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.250 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.114 | 0.323 |
| Number of reflections | 4325 | |
| <I/σ(I)> | 15.417 | |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M imidazole, pH 6.5, 600 mM sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
