3GV1
Crystal structure of disulfide interchange protein from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.329, 76.777, 64.305 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 26.040 - 2.000 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.124 | 0.668 |
| Number of reflections | 26022 | |
| <I/σ(I)> | 15.8 | 3.6 |
| Completeness [%] | 98.4 | 95.5 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.0 M DL-Malic acid pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
