3GTO
Backtracked RNA polymerase II complex with 15mer RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.851, 222.804, 194.977 |
Unit cell angles | 90.00, 101.98, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.000 |
R-factor | 0.26973 |
Rwork | 0.269 |
R-free | 0.28979 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gtg |
RMSD bond length | 0.028 |
RMSD bond angle | 1.790 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 54082 | |
<I/σ(I)> | 7.7 | 2 |
Completeness [%] | 90.4 | 77.5 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM Dioxane, 10mM DTT, 9-11% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM Dioxane, 10mM DTT, 9-11% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM Dioxane, 10mM DTT, 9-11% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2HPO4/NaH2PO4 | ||
2 | 1 | 1 | Dioxane | ||
3 | 1 | 1 | DTT | ||
4 | 1 | 1 | PEG 6000 | ||
5 | 1 | 2 | (NH4)2HPO4/NaH2PO4 | ||
6 | 1 | 2 | Dioxane | ||
7 | 1 | 2 | DTT | ||
8 | 1 | 2 | PEG 6000 |