3GT5
Crystal structure of an N-acetylglucosamine 2-epimerase family protein from Xylella fastidiosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.401, 72.052, 99.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.445 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.421 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.105 | 0.420 |
| Number of reflections | 46992 | |
| <I/σ(I)> | 11.8 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 294 | 100mM Bis-Tris pH 5.5, 25% PEG 3350, 200mM Magnesium chloride hexahydrate, VAPOR DIFFUSION, temperature 294K |
