3GT3
Structure of proteinase K with the mad triangle B3C
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 106 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9129, 0.9197, 0.9202, 0.9204 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.840, 67.840, 101.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 1.500 |
R-factor | 0.143 |
Rwork | 0.143 |
R-free | 0.18400 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.024 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.068 | 0.280 |
Number of reflections | 38897 | |
<I/σ(I)> | 24.9 | 8.3 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 14.1 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.1M TRIS, 1.28M ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |