3GT2
Crystal Structure of the P60 Domain from M. avium paratuberculosis Antigen MAP1272c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 97 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.715, 53.212, 38.325 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 22.675 - 1.750 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.20080 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.072 | 0.216 |
| Number of reflections | 13278 | |
| <I/σ(I)> | 9.446 | |
| Completeness [%] | 96.1 | 78.1 |
| Redundancy | 3.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 273 | 0.1 M BisTris-HCl, 1.6 M Ammonium Sulfate, 4% (v/v) PEG 400, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
