3GSL
Crystal structure of PSD-95 tandem PDZ domains 1 and 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.244, 62.511, 59.342 |
| Unit cell angles | 90.00, 99.25, 90.00 |
Refinement procedure
| Resolution | 32.310 - 2.050 |
| R-factor | 0.2209 |
| Rwork | 0.214 |
| R-free | 0.28146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2I1N 2fe5 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.058 | 0.349 |
| Number of reflections | 25616 | |
| <I/σ(I)> | 21.2 | 3.1 |
| Completeness [%] | 98.1 | 96.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 35% v/v 2-methyl-2,4-pentanediol, 0.1M Tris-HCl pH 7.0. 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
