3GSI

Crystal structure of D552A dimethylglycine oxidase mutant of Arthrobacter globiformis in complex with tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.072
Spacegroup name	C 2 2 2
Unit cell lengths	71.215, 224.548, 119.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.794 - 2.000
R-factor	0.1529
Rwork	0.150
R-free	0.20150
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	39.809
High resolution limit [Å]	2.000
Number of reflections	62960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	15% PEG MME 5000, 0.1M Hepes pH 7.5, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K