3GSB
CRYSTAL STRUCTURE OF GLUTAMATE-1-SEMIALDEHYDE AMINOMUTASE IN COMPLEX WITH GABACULINE
Replaces: 3GSAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.600, 108.600, 123.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.25000 |
| Starting model (for MR) | GSAT WILD-TYPE (NATIVE) STRUCTURE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.980 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.114 * | |
| Total number of observations | 57653 * | |
| Number of reflections | 18662 | |
| <I/σ(I)> | 6.2 | 1.8 |
| Completeness [%] | 98.0 | 96.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 4 * | Hennig, M., (1994) J.Mol.Biol., 242, 591. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.6-6 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | magnesium acetate | 80 (mM) | |
| 4 | 1 | drop | PEG10000 | 7.8 (%) | |
| 5 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 6 | 1 | reservoir | PEG10000 | 19.5 (%) |
