3GS9

Crystal structure of prophage tail protein gp18 (NP_465809.1) from Listeria monocytogenes EGD-e at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162
Spacegroup name	H 3
Unit cell lengths	135.575, 135.575, 59.172
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.689 - 1.700
R-factor	0.189
Rwork	0.187
R-free	0.22300
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.394
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.689	28.689	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.085	0.064	0.498
Total number of observations	128282	1415	9519
Number of reflections	44621
<I/σ(I)>	9.8	7.1	1.8
Completeness [%]	100.0	98.4	100
Redundancy	2.9	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.65	293	NANODROP, 10.0% PEG 1000, 0.20M Lithium sulfate, 0.1M Phosphate-citrate pH 4.65, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2	VAPOR DIFFUSION, SITTING DROP	4.36	293	NANODROP, 11.50% PEG 6000, 0.1M Citric acid pH 4.36, VAPOR DIFFUSION, SITTING DROP, temperature 293K