3GS8
An all-RNA hairpin ribozyme A38N1dA38 variant with a transition-state mimic substrate strand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.820, 93.820, 134.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.370 - 2.850 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.060 | 0.508 |
| Number of reflections | 8407 | |
| <I/σ(I)> | 12.2 | 2.2 |
| Redundancy | 3.81 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20.5% (w/v) PEG 2K MME, 0.10 M Na-Cacodylate, 0.25 M Li2SO4, 2.5 mM Co(NH3)6Cl3, 2 mM spermidine-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 10 | 1 | 2 | spermidine-HCl | ||
| 2 | 1 | 1 | Na-Cacodylate | ||
| 3 | 1 | 1 | Li2SO4 | ||
| 4 | 1 | 1 | Co(NH3)6Cl3 | ||
| 5 | 1 | 1 | spermidine-HCl | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Na-Cacodylate | ||
| 8 | 1 | 2 | Li2SO4 | ||
| 9 | 1 | 2 | Co(NH3)6Cl3 |
