3GS3

Structure of the N-terminal HEAT Domain of Symplekin from D. melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.97190, 0.97958
Spacegroup name	P 41 21 2
Unit cell lengths	68.336, 68.336, 139.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2068
Rwork	0.207
R-free	0.26530
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	12295
<I/σ(I)>	24.7	1.9
Completeness [%]	90.6	79.6
Redundancy	6.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	27% PEG 3350 0.425 M sodium citrate 10 mM HEPES 1 mM DTT 0.01% sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	8	295	27% PEG 3350 0.425 M sodium citrate 10 mM HEPES 1 mM DTT 0.01% sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K