3GS1
An all-RNA Hairpin Ribozyme with mutation A38N1dA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.790, 93.790, 130.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.390 - 2.850 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23300 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.190 | 3.030 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.502 | 0.502 |
| Number of reflections | 8038 | |
| <I/σ(I)> | 23.9 | 23.9 |
| Completeness [%] | 12.0 | |
| Redundancy | 8.48 | 8.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20.5% (w/v) PEG 2K MME, 0.10 M Na-Cacodylate, 0.25 M Li2SO4, 2.5 mM Co(NH3)6Cl3, 2 mM spermidine-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 10 | 1 | 2 | spermidine-HCl | ||
| 2 | 1 | 1 | Na-Cacodylate | ||
| 3 | 1 | 1 | Li2SO4 | ||
| 4 | 1 | 1 | Co(NH3)6Cl3 | ||
| 5 | 1 | 1 | spermidine-HCl | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Na-Cacodylate | ||
| 8 | 1 | 2 | Li2SO4 | ||
| 9 | 1 | 2 | Co(NH3)6Cl3 |
