3GRL

Crystal Structure of the Monomer of the p115 Tether Globular Head Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	77
Collection date	2005-12-08
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	155.629, 174.715, 87.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.410 - 2.000
R-factor	0.181
Rwork	0.178
R-free	0.20400
RMSD bond length	0.012
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	79589
<I/σ(I)>	34.3
Completeness [%]	98.7	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.2 M NaH2PO4/K2HPO4, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.2 M NaH2PO4/K2HPO4, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	1.2 M NaH2PO4/K2HPO4, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K