3GRE
Crystal structure of Saccharomyces cerevisiae Vps15 WD repeat domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.493, 85.493, 132.821 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.440 - 1.800 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.524 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.527 |
Number of reflections | 50964 | |
<I/σ(I)> | 36.003 | 1.6 |
Completeness [%] | 96.5 | 71.2 |
Redundancy | 10.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | macroseeding into microbatch | 6.5 | 291 | 0.7 M ammonium malonate, 0.05 M sodium acetate, 2% 2-propanol, pH 6.5, macroseeding into microbatch, temperature 291K |