3GRA
Crystal structure of AraC family transcriptional regulator from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-17 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 49.852, 71.876, 104.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.300 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.109 | 0.399 |
| Number of reflections | 17143 | |
| <I/σ(I)> | 12.8 | 3.54 |
| Completeness [%] | 98.8 | 92.6 |
| Redundancy | 5.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Magnesium Chloride hexahydrate, 0.1M Tris HCl pH 8.5, 25%w/v Polyethylene Glycol 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
