3GR5
Periplasmic domain of the outer membrane secretin EscC from enteropathogenic E.coli (EPEC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.645, 90.645, 133.422 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.700 - 2.050 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.102 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.700 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 20942 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, 2.0 M Ammonium sulfate + 0.1 M sodium acetate pH 4.5 or Bis-Tris pH 6.5 |
