3GR1

Periplasmic domain of the T3SS inner membrane protein PrgH from S.typhimurium (fragment 170-392)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	C 2 2 21
Unit cell lengths	79.607, 188.748, 305.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.500 - 2.800
Rwork	0.247
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gr0
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	152.500
High resolution limit [Å]	2.800
Number of reflections	55188
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		16% (w/v) PEG 3350 + 0.2 M tri-ammonium citrate + 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP