3GR0
Periplasmic domain of the T3SS inner membrane protein PrgH from S.typhimurium (fragment 170-362)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 31 |
| Unit cell lengths | 53.257, 53.257, 282.527 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 39588 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 30% (w/v) PEG 1500 + 0.1M lithium sulfate + 0.1M Tris 8.5 + 0.109M octanoyl-n-hydroxyethylglucamide, VAPOR DIFFUSION, HANGING DROP |
