3GQX

Pyrococcus Horikoshii NOP5 RNA Binding Domain from a twinned crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2006-11-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.502
Spacegroup name	P 41
Unit cell lengths	73.604, 73.604, 114.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.682 - 2.500
R-factor	0.254
Rwork	0.252
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gqu
RMSD bond length	0.004
RMSD bond angle	0.865
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.6)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	23.682
High resolution limit [Å]	1.957
Number of reflections	20529
Completeness [%]	84.0
Redundancy	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	290	500MM POTASSIUM IODIDE, 2% PEG 3350, pH 6.8, vapor diffusion, hanging drop, temperature 290K