3GQS
Crystal structure of the FHA domain of CT664 protein from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.387, 86.475, 94.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.770 - 2.200 |
| R-factor | 0.19675 |
| Rwork | 0.194 |
| R-free | 0.24419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ff4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.770 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.529 | |
| Number of reflections | 13892 | |
| <I/σ(I)> | 24.379 | 1.3 |
| Completeness [%] | 98.3 | 89 |
| Redundancy | 6.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M Na di-Hydrogen phosphate, 20% PEG 3350, VAPOR DIFFUSION, temperature 293K |
