3GQM
Crystal structure of Cell Inhibiting Factor (Cif) from Burkholderia pseudomallei (CifBp)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-06 |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.893, 77.744, 115.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.010 - 2.100 |
| R-factor | 0.21138 |
| Rwork | 0.209 |
| R-free | 0.25883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gqj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.607 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0061) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.010 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 29440 | |
| <I/σ(I)> | 20.2 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 10 % (w/v) PEG 8000, 100 mM HEPES 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
