3GQH

Crystal Structure of the Bacteriophage phi29 gene product 12 C-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	81.144, 84.158, 86.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.080 - 1.800
R-factor	0.229
Rwork	0.228
R-free	0.25600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.197
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.080	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.087	0.438
Number of reflections	55084
<I/σ(I)>	29.1	7
Completeness [%]	99.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100mM Bis-Tris at pH 7.0, 20% PEG4K and 10% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K