3GQG
Crystal structure at acidic pH of the ferric form of the Root effect hemoglobin from Trematomus bernacchii.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.720, 94.780, 61.720 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 13.900 - 1.730 |
| R-factor | 0.156 |
| Rwork | 0.152 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2H8F |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.900 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.043 | 0.175 |
| Number of reflections | 69281 | |
| Completeness [%] | 94.1 | 88.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6 | 298 | Crystallization of oxidized Hb from trematomus bernacchii was carried out in capillary at pH6 and room temperature by liquid diffusion technique (final crystallization conditions were 1.5 mg/ml protein and 8% w/v MPEG 5K)., pH 6.0, LIQUID DIFFUSION, temperature 298K |
