3GQ7

Crystal Structure of the Bacteriophage Phi29 Gene Product 12 N-terminal Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-21
Detector	MAR CCD 165 mm
Wavelength(s)	1.07
Spacegroup name	H 3 2
Unit cell lengths	89.879, 89.879, 585.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.810 - 2.050
R-factor	0.159
Rwork	0.158
R-free	0.19000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.227
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.077	0.046	0.315
Number of reflections	58072
<I/σ(I)>	28.259
Completeness [%]	99.8	99.1	99.6
Redundancy	8.3	7.7	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	298	100mM Tris-base at ~pH10.5 and 8% PEG3K, vapor diffusion, hanging drop, temperature 298K