3GQ5
Sequence-matched MutM Interrogation Complex 5 (IC5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.240, 94.443, 104.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.900 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22000 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.454 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.061 | 0.033 | 0.520 |
| Number of reflections | 35968 | ||
| <I/σ(I)> | 29.259 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 7.2 | 6.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8K | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 2 | PEG 8K | ||
| 5 | 1 | 2 | sodium cacodylate | ||
| 6 | 1 | 2 | glycerol |
