3GQ3
MutM encountering an intrahelical 8-oxoguanine (oxoG) lesion in EC5-loop deletion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.085, 92.411, 105.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 1.830 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20900 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
| Rmerge | 0.061 | 0.035 | 0.340 |
| Number of reflections | 38683 | ||
| <I/σ(I)> | 21.938 | ||
| Completeness [%] | 96.7 | 99.2 | 77.3 |
| Redundancy | 4.7 | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8K | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 2 | PEG 8K | ||
| 5 | 1 | 2 | sodium cacodylate | ||
| 6 | 1 | 2 | glycerol |
