3GQ1
The structure of the caulobacter crescentus clpS protease adaptor protein in complex with a WLFVQRDSKE decapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5416 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.533, 53.905, 45.039 |
| Unit cell angles | 90.00, 110.33, 90.00 |
Refinement procedure
| Resolution | 31.444 - 1.496 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.046 | 0.040 | 0.130 |
| Number of reflections | 21297 | ||
| <I/σ(I)> | 47.864 | ||
| Completeness [%] | 87.6 | 97.2 | 46.1 |
| Redundancy | 7.2 | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 300 | 0.1 M bis-tris pH 5.5, 0.2 M MgCl2, 20% PEG 3350, vapor diffusion, temperature 300K |
