3GQ0
The structure of the Caulobacter crescentus clpS protease adaptor protein - apo structure with no peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.667, 38.479, 62.560 |
Unit cell angles | 90.00, 88.42, 90.00 |
Refinement procedure
Resolution | 32.772 - 2.066 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.26400 |
Starting model (for MR) | 3dnj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.712 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.141 | 0.095 | 0.435 |
Number of reflections | 7983 | ||
<I/σ(I)> | 22.816 | ||
Completeness [%] | 98.2 | 98.8 | 94.5 |
Redundancy | 10.5 | 10.5 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 300 | 0.1 M bis-tris pH 5.5, 0.025 M MgCl2, 14% PEG 3350, vapor diffusion, temperature 300K |