3GPY
Sequence-matched MutM Lesion Recognition Complex 3 (LRC3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-12 |
Detector | ADSC |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.904, 95.207, 102.238 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.620 - 1.850 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.18600 |
Starting model (for MR) | 1r2y protein part only |
RMSD bond length | 0.012 |
RMSD bond angle | 1.391 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.130 | 0.079 | 0.631 |
Number of reflections | 37987 | ||
<I/σ(I)> | 13.179 | ||
Completeness [%] | 99.8 | 98.5 | 100 |
Redundancy | 8.4 | 8.1 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8K | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 2 | PEG 8K | ||
5 | 1 | 2 | sodium cacodylate | ||
6 | 1 | 2 | glycerol |