3GPX
Sequence-matched MutM Interrogation Complex 4 (IC4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-20 |
Detector | ADSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.188, 93.541, 104.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.490 - 1.780 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.21800 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.374 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
High resolution limit [Å] | 1.780 | 3.830 | 1.780 |
Rmerge | 0.055 | 0.032 | 0.485 |
Number of reflections | 43267 | ||
<I/σ(I)> | 26.142 | ||
Completeness [%] | 99.2 | 93.2 | 99.9 |
Redundancy | 5.5 | 5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8K | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 2 | PEG 8K | ||
5 | 1 | 2 | sodium cacodylate | ||
6 | 1 | 2 | glycerol |