3GPV
Crystal structure of a transcriptional regulator, MerR family from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.091, 39.175, 52.283 |
| Unit cell angles | 85.64, 85.13, 78.73 |
Refinement procedure
| Resolution | 37.260 - 1.900 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.260 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.142 | 0.432 |
| Number of reflections | 21114 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 90.7 | 49.1 |
| Redundancy | 8.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Magnesium chloride hexahydrate 0.1M Tris hydrochloride 30% Polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
